1. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 5 µL of each extracted sample was used for Picogreen analysis. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Catalog Number. Bioz Stars score: 86/100, based on 1 PubMed citations. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. 10 x 100 µL. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Lambda DNA was used to generate the. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. VIEWS. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). Quant-iT PicoGreen dsDNA Assay Kit. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. 50 pg to 2 µg. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. 9. Blog May 11, 2021. 5mL. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . 50 pg to 2 µg. Figure 1. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. no. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. • Suitable for use in 96- and 384-well plate formats. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. Unit Size. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Average 86 stars, based on 1. Mix by gently vortexing for 5 seconds. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. • Suitable for use in 96- and 384-well plate formats. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 0. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. ” application on Lunatic and compared to Qubit and. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Rapidly detect as little as 0. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 0 ml 200x in DMSO b. 99 to $1999. QuantiFluor® ONE dye sensitivity range is 0. Briefly, 2 µL of sample was diluted in 498 µL TE. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. PicoGreen dsDNA quantitation reagents are. in the assay in order to reduce waste and still achieve comparable results. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Description. 99. 99 to $1999. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Rapidly detect as little as 0. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 10. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). Easy to use UV absorbance alternative assay that can be. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Quant-iT PicoGreen dsDNA Reagent. Cloning, expression and proteins purification. 9 Q33230 9 500 28. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Volume of 20xTE = final volume/20;Application tip demonstrating that with Molecular Devices SpectraMax® microplate textbooks and the Quant-iT PicoGreen assay, users can reliably measure contents as low when 50 pg/mL of double-stranded DNA. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Add 5uL of PicoGreen reagent for every 1mL of buffer. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Assay Kit. Catalog Number. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. 2. In case dsDNA standards are available only the quantification reagent can be used. 参考資料. Highly selective for dsDNA over ssDNA or RNA. Picogreen assay kit (Invitrogen, P7589) a. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. 2022. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Quantification and normalization of double stranded DNA with minimal set-up time. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 05 to consider statistical significance. P11495. Prepare only the amount of 1:25 diluted PicoGreen required for that run. Unit Size. 5 ng/mkl – 100 mg/ml. The Quant-iT PicoGreen dsDNA. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. No. 产品概述. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Unit Size. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. • Specific for dsDNA in the presence of RNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. This sensitivity exceeds that achieved with the Hoechst 33258. 2009;49(4):997–1002. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Quantitation Range. Picogreen assays are compatible with most microplate readers and fluorometers. お問い合わせ一覧 . • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. The The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Showing 4 of 4. '. Picogreen assays are compatible with most microplate readers and fluorometers. For duplicate-well droplet. Picogreen assays are compatible with most microplate readers and fluorometers. Abstract. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Catalog number: P7581. Results: DNA concentration values were measured by the “DNA Mamm. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. Picogreen assays are compatible with most microplate readers and fluorometers. 50 pg to 2 µg. 3. Picogreen assays are compatible with most microplate readers and fluorometers. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. Quant-iT™ PicoGreen ® dsDNA Assay Kit. 04 corresponds to 2 μ g/mL dsDNA solution. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Control samples were not lysed and consisted of free pDNA. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 95 mL TE for the . Description. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. No. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 製品概要. Picogreen assays are compatible with most microplate readers and fluorometers. 3. Coleen - 06 Oct. 12. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. Highly selective for dsDNA over ssDNA or RNA. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. This was possible since, due to the low height of the light ray of only 8. The assay is performed at room temperature, and the signal is stable for 3 hours. 2. Mix PicoGreen and DNA by pippetting up and down 5-10 times. high-range. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Catalog Number. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. Price from $9. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. 2. Revised: 10–June–2008 | MP 07581 Table 1. Rapidly detect as little as 0. 21 DiB) [22]. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. 2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. P7589. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. quant it picogreen dsdna assay kit , 2023-10. n = 3. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit a. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. One microgram of DNA was sheared using an M220. g. Three kits covering a wide range of dsDNA concentrations. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Description. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. • Specific for dsDNA in the presence of RNA. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT dsDNA BR buffer (Component B) 250 ml c. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). 99 to $1999. Skip to main list 美谷分子仪器（上海）有限公司. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Rapidly detect as little as 0. 2023-11. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. The original sequencing data. Buy from Supplier. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Quant-iT PicoGreen dsDNA Assay Kit. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Thermo Fisher quant it picogreen dsdna quantitation assay kit. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quantitation using Quant-iT Assay Kits. 86. Bioz Stars score: 86/100, based on 1 PubMed citations. 1 mL kit. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. 5. Buy from Supplier. Rapidly detect as little as 0. 3. 10pg/µl vs. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. P11495. Rapidly detect as little as 0. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). high-range. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Katalognummer: P11496. • Suitable for use in 96- and 384-well plate formats. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. Technical Support Customer Service. PicoGreen dsDNA quantitation reagents are. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. Calibrator MPO. Bioz Stars score: 86/100, based on 1 PubMed citations. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 実験してみた – PicoGreenをプレートリーダーで使用してみた. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. Touch devices users use touch & swipe gestures. Figure 8. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2–100 ng: 2-1,000 ng: 0. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. High tolerance for contaminants. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). According to the obtained results, the assay presents a linear correlation in a wide range of 0. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. P11495. Picogreen assays are compatible with most microplate readers and fluorometers. For Research Use Only. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). 2–100 ng Quant-iT dsDNA HS Assay Kit 0. PicoGreen dsDNA quantitation reagents are. Materials: 1. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). • Specific for dsDNA in the presence of RNA. ” J Pharm Biomed Anal. 50 pg to 2 µg. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. 5 ng/µL. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Product Type. Common contaminants such as salts, free nucleotides, solvents,Product Description. to each well. Picogreen assays are compatible with most microplate readers and fluorometers. 2. assay. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). 激发/发射. quant it picogreen dsdna assay kit reagents. • Suitable for use in 96- and 384-well plate formats. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. • Specific for dsDNA in the presence of RNA. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 995 mL TE for the . Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. • Suitable for use in 96- and 384-well plate formats. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. Picogreen assays are compatible with most microplate readers and fluorometers. 适用于（设备）. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. 99. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 3,201 3. Absorbance-based measurements employ the DNA as it is and volumes of 100 µl, 50 µl, and 2 µl were placed into a 96 well, 384 well, or LVis plate, respectively. The kit contains. Catalog Number. PicoGreen dsDNA quantitation reagents are. Rapidly detect as little as 0. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. • Specific for dsDNA in the presence of RNA. Thermo Fisher quant it picogreen dsdna assay kit. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. No. Three kits covering a wide range of dsDNA concentrations. 2 ng to 100 ng of DNA in the assay tube. Sample fluorescence was. 2 to 100 ng) Assay Kit were used with a Qubit 3. PicoGreen dsDNA quantitation reagents are. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. • Suitable for use in 96- and 384-well plate formats. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. Fluorescence was measured at. Related applications: Nucleic Acid Quantitation. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. UV흡광 결과보다 민감도 우수. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Natural cures with herbs are. low-range. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Quant It. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Quant-iT PicoGreen dsDNA Reagent.